Determination of amino acids and polyamines in human erythrocytes. Part 2. Clinical application.
نویسنده
چکیده
1) A rapid and high sensitive method for quantitation of free amino acids and poly-. amines in human erythrocytes using high performance liquid chromatography (HPLC) was devised by the author and his colleagues. 2) Measurement of free amino acids in erythrocytes was performed on 12 patients on our ward. Marked change in amino acid pattern was observed, and there are 2 in whom the prognosis of disease was not favorable. The amino acid pattern was generally constant in the remaining 10 patients. 3) The polyamine levels in erythrocytes of 15 patients who had undergone surgical operation were determined, and it was noted that the value began to incre::ise from immediately after surgery in those whose postoperative course was good, and peaked 7 to 10 days after the operation, after which it gradually reverted to the preoperation level. In those who developed complications after operation, the pattern was different from Pattern 1. 4) No difference in polyamine value could be demonstrated between 9 cancer patients and 7 non-cancer patients. 5) A correlation between polyamine values in erythrocytes and erythrocyte aging was observed with the value being higher in young erythrocytes, i.e. reticulocytes, and low in those about to break-down. Thus, it was considered that if hematopoietic action was accelerated due to bleeding during and after operation, the polyamine values would be elevated, but if hematopoiesis was not active, the polyamine values would be low. 6) The Spm value in leucocytes was comparatively higher than that in erythrocytes, and this finding was also confirmed in clinical cases. 127 INTRODUCTION values precede increase of RNA and cell division in actively proliferating tissue. Amino acids are used as material for protein synthesis, and is administered by intra venous and enteral hyperalimentation. On the other hand, polyamines are synthesized from ornithine and s-adenosyl-methionine, but there is a correlation in volume between them and nucleic acid-protein synthesis in tissue. It has been suggested that polyamines serve as a priming agent for cell proliferation, and increase in their Russel et al. (1971) and Uehara et al. (1980) contended that determination of polyamines in urine and in erythrocytes of cancer patients was helpful in the establishment of diagnosis. Uehara et al. also claims that their erythrocyte value decreased after surgical operation. Therefore, it is considered to be an important substance from the clinical viewpoint. In our previous report19>, we described our analytical meth-
منابع مشابه
Evaluation the Effects of Foliar Treatments of Polyamines and Some Organic Acids on Quantitative and Qualitative Traits in Some Pistachio Cultivars
Among the most important problems of pistachio are physiological disorders such as fruit abscission and production of blank nuts. Environmental stresses and inadequate nutrition during flowering time have increased these problems in recent years. In order to evaluate the effects of foliar treatments with polyamines and other compounds (amino acids and ascorbic acid) on quantitative and qualitat...
متن کاملPromoted growth and improved quality of Gerbera jamesonni L. flowers using exogenous application of amino acids
Commercially available amino acids mixtures have several advantages such as enhancing assimilation of fertilizer, facilitating uptake of water and nutrients and improving photosynthesis of plants. To investigate the response of gerbera (Gerbera jamesonni L.) flowers ‘Saltino’ to foliar application of a mixture of 19 essential amino acids (0.25, 0.50 and 0.75 mg L-1) and ammonium nitrate (200 mg...
متن کاملApplication of the Freundlich Langmuir Temkin and Harkins-Jura adsorption isotherms for some amino acids and amino acids complexation with manganese kn(ll) on carbon nanolube
In lb is research, adsorption of some amino acids and their complenation with manganese (II) ion on carbonnanotube (MWCNT) by using of four relations Langmuir, Freundlich. Temkin and Harkins -Jura imitherms wasinvestigated. From this relations. Freudlich and Temkin relations predict good equilibrium diagram in, isothermcondition. we could compute the theoretical constants by excel software. Hy ...
متن کامل[Sensitive determination of reactive oxygen species by chemiluminescence methods and their application to biological samples and health foods].
Sensitive and selective methods, based on chemiluminescence reactions, were introduced for determination of reactive oxygen species (ROS) and their applications to biological samples and health foods. First, a sensitive method for determination of H(2)O(2) by peroxyoxalate chemiluminescence (PO-CL) was developed. This method could be applied to determine small amounts of H(2)O(2) in cola drinks...
متن کاملSimultaneous Determination of Different Polyamines and their Mono-Acetylated Derivatives in Gastric Tissue by HPLC with Post-Column Derivatization
A simple and highly sensitive procedure is described enabling the simultaneous determination of biogenic polyamines (PAs) and their related monoacetyl derivatives in stomach tissue. The method is based on HPLC using octane sulfonate as an ion-pairing agent employed in acetate buffers at pH 4.5. The application is accompanied with fluorescence detection followed by post-column derivatization wit...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Hiroshima journal of medical sciences
دوره 32 1 شماره
صفحات -
تاریخ انتشار 1983